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Elastic properties of different cell types and cell structures
Petri P. Lehenkari, Guillaume T. Charras, Stephen A. Nesbitt and Mike A. Horton
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Table 3. Elastic properties of different cell types and cell structures (tab003mhu) |
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| Sample type | Elastic modulus (kPa) | Refs |
| Cell | ||
| Living cells | 1–100 | 45 |
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Rat liver macrophage |
1–10 | 81 |
| Lung carcinoma cell | 13–150 | 82 |
| Human platelet | 1–50 | 83 |
| Embryonic carcinoma cell | 3.8 | 47 |
| Vinculin-deficient F9 embryonic carcinoma cell | 2.5 | 47 |
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Chicken cardiocyte |
5–200 | 84 |
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Endothelial cell |
2 (at cell centre) | 85 |
| Fibroblast-like cell | 2.23 (at cell centre) | 46 |
|
Organelle |
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| Nuclear region of rat atrial mycocytes | 500–670 | 72 |
| Secretory granules from rat mast cells | 37–4300 | 86 |
| Stress fibres | 200 | 84 |
| ‘Stable’ edge of a fibroblast | 12 | 75 |
| Leading edge of a fibroblast | 3–5 | 75 |
| Pseudo-nucleus of a platelet | 4 | 83 |
| Cortex of a platelet | 50 | 83 |
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References cited in Table 3 45 Radmacher, M. (1997) Measuring the elastic properties of biological samples with the AFM. IEEE Eng Med Biol Mag 16, 47-57, PubMed 46 Wu, H.W., Kuhn, T. and Moy, V.T. (1998) Mechanical properties of L929 cells measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking. Scanning 20, 389-397, PubMed 47 Goldmann, W.H. et al. (1998) Differences in elasticity of vinculin-deficient F9 cells measured by magnetometry and atomic force microscopy. Exp Cell Res 239, 235-242, PubMed 72 Shroff, S.G., Saner, D.R. and Lal, R. (1995) Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy. Am J Physiol 269, C286-292, PubMed 75 Rotsch, C., Jacobson, K. and Radmacher, M. (1999) Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy. Proc Natl Acad Sci U S A 96, 921-926, PubMed 81 Fritz, M. et al. (1993) Actin binding to lipid-inserted alpha-actinin. Biophys J 65, 1878-1885, PubMed 82 Weisenhorn, A.L. et al. (1993) Deformation and height anomaly of soft surfaces studied with an AFM. Nanotechnology 4, 106-113 83 Radmacher, M. et al. (1996) Measuring the viscoelastic properties of human platelets with the atomic force microscope. Biophys J 70, 556-567, PubMed 84 Hofmann, U.G. et al. (1997) Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope. J Struct Biol 119, 84-91, PubMed 85 Braet, F. et al. (1998) Imaging surface and submembranous structures with the atomic force microscope: a study on living cancer cells, fibroblasts and macrophages. J Microsc 190, 328-338, PubMed 86 Parpura, V. and Fernandez, J.M. (1996) Atomic force microscopy study of the secretory granule lumen. Biophys J 71, 2356-2366, PubMed |