|
|
|
|
|
|
|
|
|
|
Effects of cytoskeleton-disrupting drugs on the material properties of cells
Petri P. Lehenkari, Guillaume T. Charras, Stephen A. Nesbitt and Mike A. Horton
| Table 4. Effects of cytoskeleton-disrupting drugs on the material properties of cells (tab004mhu) | |||
| Cytoskeletal-disrupting drug | Effect on cytoskeleton | Effect on elastic modulus | Refs |
| Cytochalasin D | Actin filament in actin cytoskeleton disrupted | Decreased by up to threefold | 46, 81, 84 |
| Nocodazole, colcemid | Microtubular network disrupted | Increased | 46 |
| Glutaraldehyde | Cytoskeletal elements crosslinked | Increased from 1 kPa for living cells to >100 kPa on fixation | 85, 46 |
|
Protein crosslinking agent (DTSSP) |
Receptors on cell surface crosslinked | Increased (no effect, after reduction of disulphide bonds) | 46 |
| Concanavalin A | Bound to cell-surface glycoproteins | Increased twofold | 46 |
|
Pore-forming protein |
Pores formed in cell membrane |
Decreased
|
87 |
|
References cited in Table 4 46 Wu, H.W., Kuhn, T. and Moy, V.T. (1998) Mechanical properties of L929 cells measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking. Scanning 20, 389-397, PubMed 81 Fritz, M. et al. (1993) Actin binding to lipid-inserted alpha-actinin. Biophys J 65, 1878-1885, PubMed 84 Hofmann, U.G. et al. (1997) Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope. J Struct Biol 119, 84-91, PubMed 85 Braet, F. et al. (1998) Imaging surface and submembranous structures with the atomic force microscope: a study on living cancer cells, fibroblasts and macrophages. J Microsc 190, 328-338, PubMed 87 Vinckier, A. and Semenza, G. (1998) Measuring elasticity of biological materials by atomic force microscopy. FEBS Lett 430, 12-16, PubMed |