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Expert
Reviews in Molecular Medicine: http://www.expertreviews.org/
Accession information: Vol. 7; Issue 19; 20 September 2005 Abstract
PDF
Examples of improved feeders and feeder-free methods for derivation of human embryonic stem cells
Louise A. Hyslop, Lyle Armstrong, Miodrag Stojkovic and Majlinda Lako
| Table 1. Examples of improved feeders and feeder-free methods for derivation of human embryonic stem cells | ||
| Culture conditions | Details | Refs |
| Feeder cells | Human fetal muscle and skin | 99 |
| Human adult fallopian tubal epithelial layer | 99 | |
| Human foreskin fibroblasts | 100, 101 | |
| Human adult skin fibroblasts | 102 | |
| Human adult endometrial cells | 103 | |
| Human adult breast parenchyma cells | 103 | |
| Human embryonic fibroblasts from hESCs | 104, 105 | |
| Human placental fibroblasts | 10 | |
| Feeder-free substrates and defined media | Matrigel with conditioned medium from MEFs | 106 |
| Laminin with conditioned medium from MEFs | 106 | |
| Fibronectin, and medium supplemented with TGF-b, LIF and FGF-2 | 107 | |
| Human serum supplemented with conditioned medium from hESC-derived fibroblasts | 108 | |
| Matrigel, and medium supplemented with FGF-2 and noggin | 8, 29 | |
| Laminin, and medium supplemented with activin A, nicotinamide and KGF | 9 | |
| Extracellular matrix from MEFs with conditioned medium from MEFs | 7 | |
| Abbreviations: FGF-2, basic fibroblast growth factor; hESCs, human embryonic stem cells; KGF, keratinocyte growth factor; LIF, leukaemia inhibitory factor; MEFs, mouse embryonic fibroblasts; TGF-b, transforming growth factor b. | ||
| References cited
in Table 1
7 Klimanskaya, I. et al. (2005) Human embryonic stem cells derived without feeder cells. Lancet 365, 1636-1641, PubMed 8 Wang, G. et al. (2005) Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330, 934-942, PubMed 9 Beattie, G.M. et al. (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23, 489-495, PubMed 10 Simon, C. et al. (2005) First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions. Fertil Steril 83, 246-249, PubMed 29 Xu, R.H. et al. (2005) Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2, 185-190, PubMed 99 Richards, M. et al. (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20, 933-936, PubMed 100 Hovatta, O. et al. (2003) A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 18, 1404-1409, PubMed 101 Inzunza, J. et al. (2005) Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells. Stem Cells 23, 544-549, PubMed 102 Richards, M. et al. (2003) Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. Stem Cells 21, 546-556, PubMed 103 Lee, J.B. et al. (2004) Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction 128, 727-735, PubMed 104 Xu, C. et al. (2004) Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth. Stem Cells 22, 972-980, PubMed 105 Stojkovic, P. et al. (2005) An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells. Stem Cells 23, 306-314, PubMed 106 Xu, C. et al. (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19, 971-974, PubMed 107 Amit, M. et al. (2004) Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 70, 837-845, PubMed 108 Stojkovic, P. et al. (2005) Human-serum matrix supports undifferentiated growth of human embryonic stem cells. Stem Cells 23, 895-902, PubMed |
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