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Accession information: Vol. 7; Issue 19; 20 September 2005 Abstract
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Comparison of cell-surface and molecular markers for human and mouse embryonic stem cells
Louise A. Hyslop, Lyle Armstrong, Miodrag Stojkovic and Majlinda Lako
| Table 2. Comparison of cell-surface and molecular markers for human and mouse embryonic stem cells | |||
| Marker | hESCs | mESCs | Refs |
| SSEA-1 | | + | 32, 33 |
| SSEA-3 | + | | 1, 2, 98, 109 |
| SSEA-4 | + | | 1, 2, 98, 109 |
| TRA-1-60 | + | | 1, 2, 98, 109 |
| TRA-1-81 | + | | 1, 2, 98, 109 |
| TRA-254 | + | | 109 |
| GTCM-2 | + | | 2, 110 |
| Alkaline phosphatase | + | | 98, 111 |
| TG343 | + | ? | 98, 110 |
| Oct4 | + | + | 1, 2, 38, 39, 40, 98 |
| Nanog | + | + | 44, 45, 98 |
| Rex1 | + | + | 98, 112 |
| Foxd3 | + | +a | 17, 98, 113 |
| Tert | + | + | 98, 114 |
| Sox2 | + | + | 98, 115 |
| Fgfr4 | + | + | 17 |
| Utf1 | + | + | 17 |
| Terf1 | + | + | 17 |
| Terf2 | + | + | 17 |
| Cx45 | + | + | 17 |
| Cx43 | + | + | 17 |
| Glut1 | + | + | 17 |
| Bcrp1 | + | + | 116 |
| a
Variable between cell lines. Abbreviations: Bcrp1, breast cancer resistance protein 1; Cx43/45, connexin 43/45; Fgfr4, fibroblast growth factor receptor 4; Glut1, glucose transporter 1; Foxd3, forkhead box D3; Rex1, zinc finger protein 42; Sox2, SRY-related HMG-box gene 2; SSEA, stage-specific embryonic antigen; Terf, telomeric repeat binding factor; Tert, telomerase reverse transcriptase; Utf1, undifferentiated embryonic cell transcription factor. GTCM-2, TG343 and TRA are antibodies that detect epitopes on specific proteoglycans or associated proteins. |
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| References cited
in Table 2
1 Thomson, J.A. et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147, PubMed 2 Reubinoff, B.E. et al. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18, 399-404, PubMed 17 Ginis, I. et al. (2004) Differences between human and mouse embryonic stem cells. Dev Biol 269, 360-380, PubMed 32 Vallier, L., Reynolds, D. and Pedersen, R.A. (2004) Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway. Dev Biol 275, 403-421, PubMed 33 Staal, F.J. and Clevers, H.C. (2005) WNT signalling and haematopoiesis: a WNT-WNT situation. Nat Rev Immunol 5, 21-30, PubMed 38 Palmieri, S.L. et al. (1994) Oct-4 transcription factor is differentially expressed in the mouse embryo during establishment of the first two extraembryonic cell lineages involved in implantation. Dev Biol 166, 259-267, PubMed 39 Niwa, H., Miyazaki, J. and Smith, A.G. (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24, 372-376, PubMed 40 Nichols, J. et al. (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95, 379-391, PubMed 44 Mitsui, K. et al. (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113, 631-642, PubMed 45 Chambers, I. et al. (2003) Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113, 643-655, PubMed 98 Stojkovic, M. et al. (2004) Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells 22, 790-797, PubMed 109 Henderson, J.K. et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20, 329-337, PubMed 110 Pera, M.F., Reubinoff, B. and Trounson, A. (2000) Human embryonic stem cells. J Cell Sci 113 ( Pt 1), 5-10, PubMed 111 Wobus, A.M. et al. (1984) Characterization of a pluripotent stem cell line derived from a mouse embryo. Exp Cell Res 152, 212-219, PubMed 112 Rogers, M.B., Hosler, B.A. and Gudas, L.J. (1991) Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes. Development 113, 815-824, PubMed 113 Hanna, L.A. et al. (2002) Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo. Genes Dev 16, 2650-2661, PubMed 114 Armstrong, L. et al. (2000) mTert expression correlates with telomerase activity during the differentiation of murine embryonic stem cells. Mech Dev 97, 109-116, PubMed 115 Avilion, A.A. et al. (2003) Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 17, 126-140, PubMed 116 Rao, M. (2004) Conserved and divergent paths that regulate self-renewal in mouse and human embryonic stem cells. Dev Biol 275, 269-286, PubMed |
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