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Expert
Reviews in Molecular Medicine: http://www.expertreviews.org/
Accession information: Vol. 8; Issue 1; 10 January 2006 Abstract
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Diagram of mRNA cis-splicing versus trans-splicing
Hengjun Chao and Christopher E. Walsh
Figure 1. Diagram of mRNA cis-splicing versus trans-splicing. (a) The process of cis-splicing: a single pre-mRNA, shown here containing four exons (mutation on exon 3) and three introns, is spliced together to form mature mRNA with mutation. (b) The process of trans-splicing to repair the mutation. Pre-mRNA derived from exon 2 from the endogenous pre-mRNA is shown splicing to mRNA derived from exon 3 of the pre-trans-splicing molecule (PTM), to generate a composite mRNA that now carries non-mutated exon 3. Prototype PTMs carry a binding domain (BD) that is complementary to the target intron and that tethers the PTM to a specific location within the target; a splicing sequence (ss) containing splicing elements that are equivalent to those found in cis-splicing precursors; a branchpoint (BP), which participates in the conjoining of two flanking exons; a polypyrimidine (Py) tract, which is crucial for initial recognition of an intron; and a coding domain consisting of one or more exons that are trans-spliced to the target. Both processes (cis and trans) are mediated by the spliceosome.
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