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Expert
Reviews in Molecular Medicine: http://www.expertreviews.org/
Accession information: Vol. 8; Issue 2; 17 January 2006 Abstract
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Pkd1 expression in the mouse embryo
Bradley K. Yoder, Sharon Mulroy, Hannah Eustace, Catherine Boucher and Richard Sandford
Figure 4. Pkd1 expression in the mouse embryo. A heterozygous Pkd1del17-22bgeo E12.5 mouse embryo was stained with X-gal to demonstrate the expression of Pkd1 using a ‘knocked-in’ b-galactosidase reporter gene and imaged using optical projection tomography (Ref. 31). The b-galactosidase reporter gene was fused to the Pkd1 gene so that Pkd1 expression resulted in b-galactosidase expression. The b-galactosidase substrate, X-gal, which produces a coloured product, can then be used to monitor Pkd1 expression. Pkd1 expression (green) is seen in the developing cartilage, cardiac outflow tract, smooth muscle and urogenital sinus in addition to many other tissues. Sections are taken at intervals through a whole-mount embryo to demonstrate the different sites of expression. See also the optical projection tomography of this figure (Movie 1). For further information on optical projection tomography see Ref. 116. Abbreviation: Pkd1, mouse polycystic kidney disease 1 gene.
| References cited
in Figure 4
31 Boulter, C. et al. (2001) Cardiovascular, skeletal, and renal defects in mice with a targeted disruption of the Pkd1 gene. Proc Natl Acad Sci U S A 98, 12174-12179, PubMed 116 Sharpe, J. et al. (2002) Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science 296, 541-545, PubMed |
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