Expert Reviews in Molecular Medicine: http://www.expertreviews.org/
Accession information: (99)00130-1h.htm (shortcode: fig003sjd); 6 December 1999
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Phylogenetic relationships between hepatitis E virus isolates

Shahid Jameel

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Figure 3. Phylogenetic relationships between hepatitis E virus isolates. Nucleotide sequences encoding the structural regions [open reading frame 2 (ORF2) and ORF3] of selected hepatitis E virus (HEV) isolates from different geographic locales were subjected to phylogenetic analyses (S. Jameel, unpublished). The sequences were obtained from GenBank (http://www.ncbi.nlm.nih.gov/). The Asia-1 clade includes India-1 (AF076239), India-2 (X99441), Nepal (AF051830) and Myanmar-3 (M73218) isolates; the Asia-2 clade includes China-1 (D11092), China-3 (L25547) and Pakistan (M80581) isolates; the North Africa clade includes Egypt-1 (AF051352), Egypt-2 (AF051351) and Morocco (AF065061) isolates; and the North America clade includes Mexico (M74506), US-1(AF060668) and Swine-1 (AF082843) isolates. The 2024-nucleotide sequences were first aligned using Clustal in the McVector v6.0 software package (Oxford Molecular Group, UK). Phylogenetic analyses were then carried out with algorithms in the PHYLIP v3.5 package (http://evolution.genetics.washington.edu/phylip.html), as follows: the aligned sequences were subjected to 5000 rounds of bootstrapping with SEQBOOT; the bootstrapped replicates were then subjected to either DNA parsimony analysis with DNAPARS or DNA distance analysis using DNADIST and NEIGHBOR; and unrooted consensus trees were drawn using CONSENSE and DRAWTREE with the Mexico sequence as the outgroup. The illustration shows a DNA parsimony tree; the DNA distance tree also showed identical relationships between various isolates. Results from other phylogenetic analyses using shorter stretches of nucleotide sequences from various isolates of HEV also demonstrate similar relationships (Ref. 16) (fig003sjd).

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